Educational guide for sterile handling concepts. Not cultivation advice. Follow all applicable laws and safety standards.
What Is a Still Air Box?
A Still Air Box (SAB) is a simple enclosure used to limit airflow and reduce the movement of airborne contaminants during sensitive lab work. By working within a confined, relatively motionless airspace, researchers can perform tasks (e.g., slide prep, transfers between sterile containers) with fewer contaminants drifting into open vessels or onto implements.
SAB vs Laminar Flow Hood
- SAB: Relies on still air. Inexpensive, accessible, and effective when used with disciplined technique.
- Laminar Flow Hood: Uses filtered, directional airflow to create a clean working zone. Offers higher throughput and comfort but at greater cost and complexity.
Pros and Cons
SAB Pros:
- Low cost and easy to assemble from common materials.
- Portable and suitable for small workspaces.
SAB Cons:
- Limited maneuvering space.
- Requires very deliberate movements to maintain clean conditions.
Using a SAB Safely and Effectively
- Prep the space: Clean the interior, place materials in logical order, and minimize unnecessary items.
- Personal prep: Wear gloves, tie back hair, avoid loose sleeves; wipe gloves and surfaces with alcohol and let evaporate.
- Movement discipline: Keep motions slow and deliberate; avoid rapid hand movements that cause air currents.
- Flame sterilization: If flame is used, manage heat safely and position tools to reduce risk.
- Lid etiquette: Keep lids and caps closed as much as possible; open just enough, for just long enough, to perform the task.
Frequently Asked Questions
Do I need a flow hood to do microscopy or basic sterile work?
Not necessarily. Many researchers begin with a SAB and achieve clean results by refining technique.
How big should a SAB be?
Large enough to comfortably fit your tools and hands without bumping into surfaces, but not so large that it becomes unwieldy.
What common mistakes should I avoid?
Overcrowding the box, moving too fast, neglecting glove or surface prep, and leaving containers open longer than necessary.
Related Terms and Links
- Glossary: Laminar Flow Hood, Aseptic Technique, Airport Lid
- Guides: Complete Mycology Guide
Legal and Safety Notes
- For educational sterile technique only.
- Do not perform prohibited activities in your jurisdiction.