Definition: A monotub is a lidded container configured to balance humidity and gas exchange around a colonized bulk substrate in a simple, self-contained environment.
Typical components
- Opaque or clear tote with fitted lid.
- Passive air-exchange points (e.g., filtered openings) to avoid stale conditions.
- Even, well-aerated bulk substrate and mixed-in spawn.
- Optional liner to reduce side drying and make cleanup easier.
How a monotub works (overview)
The container, which acts as a fruiting chamber, buffers humidity while passive exchange prevents stagnation. Surface conditions—fine droplets without pooling—are a common indicator of a balanced environment. Excess condensation or dryness signals that adjustments to room airflow or tub sealing may be needed.
Pros and cons
| Pros | Cons |
|---|---|
| Simple, low-cost, scalable | Sensitive to room environment; requires observation |
| Self-contained humidity buffer | Limited fine control compared with controlled chambers |
| Easy to isolate projects | Improper sealing can favor Trich or other competitors |
Care & cleanliness
- Clean the container between uses; avoid abrasives that create micro-scratches harboring spores.
- Handle only in lawful research contexts and with clean technique.
Legal & safety: For permitted species and lawful research only. Follow local regulations and lab hygiene.